2.

DC-T cells combined with endostatin efficiently decrease multiple proangiogenic factors and augment hypoxia in tumors. (A, B) VEGF and HIF-1α expressions in tumors determined by Western blotting. β-actin served as an internal control. Data are expressed as x±s of three independent experiments. *, P<0.05 compared with control; (C) Expression of VEGF and HIF-1α in tumors determined by IHC (400×); (D) Pro/anti-angiogenic cytokines IL-6, IL-17 and IFN-γ assessed by ELISA. Data are expressed as x±s of three independent experiments. *, P<0.05 compared with control; **, P<0.01 compared with control. VEGF, vascular endothelial growth factor; HIF, hypoxia-inducible factor; IFN, interferon.