3.

DC-T cells in combination with endostatin significantly decrease immunosuppressive cells in tumor microenvironment and increase tumor-infiltrated mDC and CD8+ T cells. (A) Effect of DC-T cells (5×10⁶ cells, d 1) in combination with endostatin (15 mg/kg, d 1-14) on immunoinhibitory cells (MDSC, M1 and M2); (B) Effect of DC-T combined with endostatin on mDC and cytotoxic CD8+ T cells. The cell proportions were detected by flow cytometry. Data are expressed as x±s of three independent experiments. *, P<0.05 compared with control; **, P<0.01 compared with control. MDSC, myeloid-derived suppressor cells; M1, tumor-associated macrophages of phenotype 1; M2, tumor-associated macrophages of phenotype 2.