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. 2016 Jul 16;59(2):93–99. doi: 10.3164/jcbn.16-15

Fig. 3.

Fig. 3

l-Theanine increased GSH levels in astrocytes. (A) Effects of l-theanine on GSH content in cultured neurons and astrocytes. GSH levels were measured in cultured neurons and astrocytes after treatment with theanine (5, 50, 500 µM) or vehicle for 72 h. Data are mean ± SEM of 6 samples/experiment. *p<0.05 compared with the vehicle-treated control group. (B) Changes in GSH levels in astroglial conditioned media (GCM) after treatment with l-theanine (50, 500 µM) or vehicle for 72 h. Data are mean ± SEM of 6 samples/experiment. **p<0.01, ***p<0.001 compared with the vehicle-treated control group. (C) Effects of treatment with l-theanine (0, 5, 50, 500 µM) for 72 h on GCL in cultured astrocytes. Protein levels were examined by western blot analysis. Data are mean ± SEM of 6 samples/experiment.