Fig. 2.

Effect of UA on sensitivity of colon cancer cells to chemotherapeutic drugs under hypoxia

Cells were pretreated with UA (20 μmol/L) for 24 h in normoxia. Cells were then cultured under normoxic (20% O₂) or hypoxic (1% O₂) conditions with 5-FU (a) or oxaliplatin (b) for 24 h. Cell viability was evaluated using MTT assays. (c) Flow cytometry analysis of apoptotic colon cancer cells treated with 5-FU alone or combined with UA under normoxia and hypoxia. Data are expressed as mean±SD of triplicate experiments. ^* P<0.05, compared to N+5-FU; ^# P<0.05, ^### P<0.005 compared to hypoxia. N: normoxia; H: hypoxia