Table 1. The detailed characteristics of WRKY genes identified in willow.
| Gene | Sequence ID | Chr | Group | Ortholog | Deduced polypeptide | Introns | |||
|---|---|---|---|---|---|---|---|---|---|
| AtWRKY | PtWRKY | Length (aa) | PI | MW (kDa) | |||||
| SsWRKY1 | willow_GLEAN_10011238 | 1 | I | 33 | 17 | 583 | 7.14 | 64.7 | 4 |
| SsWRKY2 | willow_GLEAN_10019192 | 1 | IIc | 45 | 43 | 162 | 9.47 | 18.6 | 1 |
| SsWRKY3 | willow_GLEAN_10017208 | 1 | IIc | 28,71 | 29 | 584 | 9.42 | 65.6 | 4 |
| SsWRKY4 | willow_GLEAN_10017139 | 1 | I | 20 | 44 | 560 | 6.99 | 60.9 | 5 |
| SsWRKY5 | willow_GLEAN_10007860 | 1 | IIe | 35 | 45 | 445 | 5.92 | 48.4 | 2 |
| SsWRKY6 | willow_GLEAN_10003806 | 1 | I | 2 | 37,101,102 | 733 | 5.69 | 78.8 | 4 |
| SsWRKY7 | willow_GLEAN_10022392 | 2 | IId | 21 | 46,63 | 453 | 9.53 | 49.9 | 4 |
| SsWRKY8 | willow_GLEAN_10022273 | 2 | IIc | 71 | 47 | 328 | 6.89 | 37.0 | 2 |
| SsWRKY9 | willow_GLEAN_10009329 | 2 | IId | 15 | 14,94 | 339 | 9.77 | 37.5 | 2 |
| SsWRKY10 | willow_GLEAN_10009231 | 2 | IIc | 12 | 48 | 204 | 7.64 | 23.6 | 3 |
| SsWRKY11 | willow_GLEAN_10016913 | 2 | III | 30 | 6,51 | 351 | 6.27 | 39.2 | 2 |
| SsWRKY12 | willow_GLEAN_10016886 | 2 | IIc | – | 19,50 | 129 | 6.75 | 14.6 | 0 |
| SsWRKY13 | willow_GLEAN_10016883 | 2 | IIe | 22 | 23,49,78 | 352 | 5.81 | 38.3 | 2 |
| SsWRKY14 | willow_GLEAN_10019911 | 2 | IIe | – | 3 | 247 | 5.58 | 28.1 | 2 |
| SsWRKY15 | willow_GLEAN_10019925 | 2 | IIc | 23 | 13,33 | 319 | 6.46 | 35.6 | 2 |
| SsWRKY16 | willow_GLEAN_10019982 | 2 | I | 1 | 54 | 472 | 6.88 | 52.2 | 3 |
| SsWRKY17 | willow_GLEAN_10020022 | 2 | IIb | 47 | 53 | 1,081 | 5.25 | 116.8 | 17 |
| SsWRKY18 | willow_GLEAN_10025583 | 3 | IId | – | 55 | 142 | 9.60 | 16.5 | 2 |
| SsWRKY19 | willow_GLEAN_10025423 | 3 | IIe | 29 | 41 | 335 | 5.54 | 37.9 | 2 |
| SsWRKY20 | willow_GLEAN_10025378 | 3 | III | 41/53 | 21 | 342 | 5.25 | 38.4 | 2 |
| SsWRKY21 | willow_GLEAN_10008020 | 3 | IIc | 45 | 18 | 157 | 9.41 | 17.8 | 1 |
| SsWRKY22 | willow_GLEAN_10006448 | 3 | IIa | 40 | 88 | 320 | 8.38 | 35.4 | 3 |
| SsWRKY23 | willow_GLEAN_10013342 | 3 | IIc | – | 39 | 109 | 8.03 | 12.9 | 1 |
| SsWRKY24 | willow_GLEAN_10009960 | 4 | IIb | 42 | 28,79 | 604 | 6.93 | 65.3 | 5 |
| SsWRKY25 | willow_GLEAN_10017267 | 4 | IIe | 65 | 8,58 | 267 | 5.43 | 29.7 | 2 |
| SsWRKY26 | willow_GLEAN_10018559 | 4 | I | 58 | 60 | 537 | 8.72 | 58.9 | 3 |
| SsWRKY27 | willow_GLEAN_10004854 | 4 | III | 54 | 85 | 323 | 5.70 | 36.3 | 2 |
| SsWRKY28 | willow_GLEAN_10008312 | 5 | IId | – | – | 490 | 10.27 | 54.0 | 2 |
| SsWRKY29 | willow_GLEAN_10009112 | 5 | IIc | 13 | 68 | 235 | 8.70 | 26.7 | 2 |
| SsWRKY30 | willow_GLEAN_10003565 | 5 | IId | 15 | 20 | 310 | 9.48 | 34.3 | 2 |
| SsWRKY31 | willow_GLEAN_10016009 | 5 | IIc | 28,71 | 62 | 322 | 6.67 | 36.2 | 2 |
| SsWRKY32 | willow_GLEAN_10018195 | 5 | IId | 21 | 46,63 | 349 | 9.69 | 38.8 | 2 |
| SsWRKY33 | willow_GLEAN_10026833 | 6 | IId | 7 | 91 | 339 | 9.89 | 36.8 | 3 |
| SsWRKY34 | willow_GLEAN_10026721 | 6 | IIc | 49 | 34 | 287 | 5.25 | 32.1 | 2 |
| SsWRKY35 | willow_GLEAN_10026591 | 6 | I | 33 | 64 | 572 | 6.41 | 62.7 | 4 |
| SsWRKY36 | willow_GLEAN_10026566 | 6 | III | 54 | 85 | 329 | 6.13 | 36.7 | 2 |
| SsWRKY37 | willow_GLEAN_10020588 | 6 | I | 44 | 93 | 478 | 9.25 | 52.5 | 4 |
| SsWRKY38 | willow_GLEAN_10026166 | 6 | IIc | 51 | 67 | 233 | 5.03 | 26.1 | 2 |
| SsWRKY39 | willow_GLEAN_10026455 | 6 | IIa | 18/60 | 9 | 327 | 9.02 | 36.2 | 4 |
| SsWRKY40 | willow_GLEAN_10026458 | 6 | I | 32 | 15 | 413 | 8.26 | 44.9 | 3 |
| SsWRKY41 | willow_GLEAN_10008192 | 7 | IIc | 13 | 68 | 236 | 9.21 | 26.6 | 2 |
| SsWRKY42 | willow_GLEAN_10025108 | 8 | I | 3/4 | 69 | 460 | 8.80 | 50.6 | 3 |
| SsWRKY43 | willow_GLEAN_10025123 | 8 | IIc | 57 | 71 | 295 | 6.32 | 32.3 | 2 |
| SsWRKY44 | willow_GLEAN_10015641 | 8 | IIc | 48 | 70 | 357 | 6.11 | 39.9 | 2 |
| SsWRKY45 | willow_GLEAN_10008155 | 9 | IId | 15 | 20,26 | 331 | 9.57 | 36.4 | 2 |
| SsWRKY46 | willow_GLEAN_10013562 | 10 | IIc | 57 | 71 | 289 | 6.26 | 31.9 | 2 |
| SsWRKY47 | willow_GLEAN_10013586 | 10 | I | 3/4 | 72 | 490 | 8.60 | 53.7 | 3 |
| SsWRKY48 | willow_GLEAN_10004012 | 11 | IIb | 42 | 100 | 585 | 6.48 | 63.3 | 5 |
| SsWRKY49 | willow_GLEAN_10006060 | 11 | I | 20 | 44 | 607 | 7.09 | 6.6 | 6 |
| SsWRKY50 | willow_GLEAN_10007614 | 11 | IIe | 35 | 74 | 481 | 5.39 | 51.6 | 3 |
| SsWRKY51 | willow_GLEAN_10007542 | 11 | I | 2 | 37 | 734 | 6.10 | 79.7 | 4 |
| SsWRKY52 | willow_GLEAN_10013801 | 12 | IIc | – | 75 | 178 | 9.08 | 20.5 | 1 |
| SsWRKY53 | willow_GLEAN_10012158 | 13 | IId | 74 | 25 | 356 | 9.66 | 40.0 | 2 |
| SsWRKY54 | willow_GLEAN_10004417 | 13 | I | 2 | 35 | 697 | 6.52 | 76.1 | 4 |
| SsWRKY55 | willow_GLEAN_10007732 | 13 | I | 33 | 1 | 602 | 7.65 | 66.0 | 4 |
| SsWRKY56 | willow_GLEAN_10009039 | 14 | IId | 15 | 14,94 | 362 | 9.39 | 40.0 | 2 |
| SsWRKY57 | willow_GLEAN_10016668 | 14 | IIc | 12 | 48 | 180 | 8.47 | 20.7 | 3 |
| SsWRKY58 | willow_GLEAN_10016177 | 14 | IIe | 22 | 23,49,78 | 354 | 6.35 | 38.8 | 2 |
| SsWRKY59 | willow_GLEAN_10016180 | 14 | IIc | 43 | 19,50 | 193 | 9.47 | 21.7 | 1 |
| SsWRKY60 | willow_GLEAN_10016220 | 14 | III | 30 | 6 | 368 | 5.03 | 41.3 | 2 |
| SsWRKY61 | willow_GLEAN_10018940 | 14 | IIb | 42 | 28,79 | 467 | 8.78 | 50.0 | 5 |
| SsWRKY62 | willow_GLEAN_10018891 | 14 | IIc | 23 | 13,33 | 318 | 5.71 | 35.6 | 2 |
| SsWRKY63 | willow_GLEAN_10018881 | 14 | IIe | – | 80 | 263 | 5.05 | 29.7 | 2 |
| SsWRKY64 | willow_GLEAN_10020302 | 14 | IIb | 36 | – | 460 | 6.28 | 50.0 | 4 |
| SsWRKY65 | willow_GLEAN_10020380 | 14 | I | 1 | 2 | 481 | 5.98 | 52.8 | 3 |
| SsWRKY66 | willow_GLEAN_10011119 | 15 | IIb | 9 | 99 | 618 | 6.55 | 66.2 | 5 |
| SsWRKY67 | willow_GLEAN_10016438 | 15 | IIc | – | 82 | 178 | 9.35 | 20.5 | 1 |
| SsWRKY68 | willow_GLEAN_10023347 | 16 | IIa | 40 | 88 | 320 | 8.82 | 35.3 | 3 |
| SsWRKY69 | willow_GLEAN_10023447 | 16 | IIc | 45 | 18 | 178 | 9.17 | 20.1 | 1 |
| SsWRKY70 | willow_GLEAN_10023687 | 16 | III | 41/53 | 21 | 336 | 5.17 | 37.2 | 2 |
| SsWRKY71 | willow_GLEAN_10023735 | 16 | IIe | 29 | 41 | 325 | 5.54 | 36.6 | 2 |
| SsWRKY72 | willow_GLEAN_10014752 | 16 | IId | – | 55 | 338 | 9.24 | 37.9 | 2 |
| SsWRKY73 | willow_GLEAN_10009602 | 16 | IIb | 9 | 42 | 509 | 5.51 | 55.3 | 4 |
| SsWRKY74 | willow_GLEAN_10010473 | 17 | IIc | 45 | 43 | 182 | 9.92 | 20.9 | 1 |
| SsWRKY75 | willow_GLEAN_10015128 | 17 | IIb | 9 | 86 | 544 | 6.01 | 59.0 | 3 |
| SsWRKY76 | willow_GLEAN_10015184 | 17 | I | 58 | 87 | 1,044 | 8.94 | 116.1 | 11 |
| SsWRKY77 | willow_GLEAN_10005468 | 17 | IIe | 27 | 96 | 411 | 5.96 | 45.7 | 2 |
| SsWRKY78 | willow_GLEAN_10006860 | 18 | I | – | 90 | 1,593 | 8.67 | 179.0 | 10 |
| SsWRKY79 | willow_GLEAN_10006862 | 18 | IIa | 18/60 | 9 | 320 | 8.57 | 35.6 | 4 |
| SsWRKY80 | willow_GLEAN_10011608 | 18 | I | 32 | – | 528 | 5.74 | 57.8 | 4 |
| SsWRKY81 | willow_GLEAN_10004546 | 18 | IId | 7 | 7,91 | 300 | 9.80 | 32.8 | 2 |
| SsWRKY82 | willow_GLEAN_10003422 | 19 | IId | 11/17 | 24 | 339 | 9.58 | 37.1 | 2 |
| SsWRKY83 | willow_GLEAN_10011321 | 19 | III | 55 | 36,76 | 358 | 5.63 | 38.7 | 2 |
| SsWRKY84 | willow_GLEAN_10005288 | 19 | I | 33 | 4 | 597 | 6.69 | 65.6 | 4 |
| SsWRKY85 | willow_GLEAN_10002834 | N/A | IIe | 65 | 58 | 268 | 5.83 | 30.2 | 2 |
Notes:
Chr, chromosome numbers.
N/A, not available.
“–”, not detected.