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. 2016 Sep 7;4:e2435. doi: 10.7717/peerj.2435

Figure 2. MxaY regulates the lanthanide-mediated MDH switch.

Figure 2

(A) Real-time qRT-PCR was performed on RNA harvested from wild-type and ΔmxaY(FC74) M. buryatense 5GB1C strains grown in the absence of lanthanum. The values shown represent the fold change in mxaF, mxaB, and xoxF gene expression when transcript levels from the ΔmxaY strain were compared to wild-type M. buryatense 5GB1C. (B) Real-time qRT-PCR was performed on RNA harvested from the ΔmxaY mutant grown with and without lanthanum. Results shown represent the fold change in gene expression in ΔmxaY cells grown with lanthanum compared to gene expression in ΔmxaY cells grown without lanthanum. All gene expression was normalized to 16S rRNA transcript levels. Significant differences between gene expression levels in (A) and (B) were determined by using unpaired t-tests (∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05). La3+, lanthanum. (C, D) Growth curves for wild-type M. buryatense 5GB1C, ΔmxaY (FC74), ΔmxaY complemented with a wild-type version of mxaY (ΔmxaY; mxaY; FC77), and ΔmxaY complemented with the E147G version of mxaY (ΔmxaY; mxaY E147G; FC78) strain variants grown in the presence (C, filled symbols) or absence (D, empty symbols) of 30 µM lanthanum. Data represent the means from three replicates and error bars represent standard deviations.