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. 2016 Sep 7;4:e2435. doi: 10.7717/peerj.2435

Figure 3. MxaY E147G is a constitutively active version of MxaY.

Figure 3

(A) Real-time qRT-PCR was performed on RNA harvested from wild-type and MxaY E147G (FC78) M. buryatense 5GB1C strains grown in the presence of 30 µM lanthanum. The values shown represent the fold change in gene expression in MxaY E147G compared to wild-type M. buryatense 5GB1C. (B) Real-time qRT-PCR was performed on RNA harvested from MxaY E147G (FC78) cells grown in the presence or absence 30 µM lanthanum. Results represent the fold change in gene expression in cells grown with lanthanum compared to cells grown without lanthanum. All gene expression was normalized to 16S rRNA transcript levels. Significant differences between gene expression levels was determined by using unpaired t-tests (∗∗p < 0.01; ∗p < 0.05). Data represent the means from three replicates and error bars represent standard deviations. La3+, lanthanum.