Figure 3. Immunocytochemical staining for osteogenic differentiation markers.

Cells treated with PRC had stronger positive staining for Runx2 compared to the cells cultured in osteogenic medium, which was observed by day 8. Both osteocalcin and osteopontin were detected at a comparable level to the osteogenic medium. Expression of the proteins in the cytoplasm was reflected by green fluorescence and nucleus was stained blue by Hoechst. The above are representative images viewed at 10× magnification (scale bar: 100 μM).