Figure 5. Western blot analysis of protein levels in rat lung tissue. A, Concentrations of Nox4, p22phox and p47phox were determined by western blot analysis; B, Nox4/β-actin, C, p22phox/β-actin; D, p47phox/β-actin. Results of a representative experiment are shown. Ang-(1–7) down-regulated the CIH-induced elevation of Nox4, p22phox and p47phox expression. Data are reported as means±SE. NC: normoxia control group (n=8); N-A: NC and Ang-(1–7) supplemented group (n=8); uCIH: untreated chronic intermittent hypoxia group (n=8); CIH-A: CIH and Ang-(1–7) supplemented group (n=8). *P<0.05 vs NC; P<0.05 vs N-A; +P<0.05 vs uCIH (ANOVA).
