Fig. 1.
The full CC domains of MLA10, Sr33, and Sr50 are sufficient for autoactivity in planta. Indicated fragments of the MLA10, Sr33, and Sr50 proteins fused to HA or CFP were transiently expressed in N. benthamiana leaves by Agrobacterium tumefaciens infiltration along with the autoactive RGA4:HA or RGA4:CFP positive controls and the inactive rga4TYG/MHD:HA or rga4TYG/MHD:CFP or mock inoculation as negative controls. Pictures were taken 3 d (A) or 5 d (B and C) after infiltration. Equivalent results were obtained in three independent experiments.