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. 2016 Aug 23;113(36):10204–10209. doi: 10.1073/pnas.1605483113

Fig. 5.

Fig. 5.

Sr33 induces disease-resistance signaling from the cytosol of wheat cells. (A) Untransformed Fielder and two independent T1 transgenic lines per construct expressing Sr33 fused to YFPv, YFPv:NLS, YFPv:nls, YFPv:NES, or YFPv:nes plants were inoculated with Pgt strain 98-1,2,3,5,6 at the two-leaf stage. Pictures were taken 13 d after inoculation. The presence of the transgenes was determined by PCR with Sr33-specific primers. (B) Immunoblot of transgenic wheat seedlings expressing YFP-tagged proteins detected with anti-GFP.