Fig. 6.

Localization of Sr33 fusion proteins in wheat mesophyll protoplasts. (A) Linear unmixing confocal images showing the localization of Sr33 fused to the YFPv, YFPv:NLS, YFPv:nls, YFPv:NES, or YFPv:nes in transgenic wheat mesophyll protoplasts. Protoplasts were isolated from 10- to 14-d-old T1 transgenic plants and were observed within 8 h following isolation. YFP-specific fluorescence appears in green; specific vacuolar and chloroplastic autofluorescence emissions are shown in blue and red, respectively. White circles mark nuclei locations on bright-field pictures. (Scale bars, 5 µm.)