Fig. S2.
Cell-death assays in N. benthamiana. (A) The MLA10 CC1–120, Sr33 CC1–120, and Sr50 CC1–123 domains fused to HA were transiently expressed in N. benthamiana leaves. The autoactive RGA4:HA construct was used as a positive control, and the inactive rga4TYG/MHD:HA and a mock inoculation were used as negative controls. Cell death was visualized 4 d after infiltration. Equivalent results were obtained in three independent experiments. (B) The indicated Sr33 and Sr50 genomic constructs (including their native promoter sequences) or cDNA sequences (under the control of the 35S promoter) were transiently expressed in N. benthamiana leaves by Agrobacterium-mediated transformation. Cell death was visualized 4 d after infiltration. Equivalent results were obtained in three independent experiments.