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. 2016 Aug 23;113(36):10204–10209. doi: 10.1073/pnas.1605483113

Fig. S5.

Fig. S5.

Cell-death and in planta coimmunoprecipitation assays. (A) The indicated combinations of constructs fused to either the CFP or the HA tag were transiently expressed in N. benthamiana leaves. Samples were collected for coimmunoprecipitation assays 24 h after infiltration, and pictures were taken 3 d after infiltration. (B) The truncated CC domains of MLA10, Sr33, and Sr50 do not self-associate in planta. Coimmunoprecipitation was performed as described in Fig. 2. The Sr50 CC1–160 positive control was included in the experiment. (C) The full-length Sr33 protein self-associates in planta. Coimmunoprecipitation was performed as described in Fig. 2. An additional control was included to test Sr33-unspecific binding to the GFP-trap_M beads. (D) Full-length Sr33 and Sr50 homo- and hetero-associate in planta. Coimmunoprecipitation was performed as described in Fig. 2. A control was included to test Sr33- and Sr50-unspecific binding to the GFP-trap_M beads.