Confocal imaging in N. benthamiana and wheat epidermal cells. (A) CC domains fused to YFP:NLS, YFP:nls, YFP:NES, or YFP:nes were transiently expressed in N. benthamiana. Confocal images were taken 20 h after infiltration. Arrows indicate nuclei. (Scale bars, 20 μm.) (B) Confocal images showing localization of Sr33 fused to the YFPv, YFPv:NLS, YFPv:nls, YFPv:NES, or YFPv:nes in transgenic wheat leaf epidermal cells were taken on 10- to14-d-old T1 plants. YFPv fluorescence appears in green; stomatal and chloroplastic autofluorescence emissions are shown in blue and red, respectively. White arrowheads mark nuclei. (Scale bars, 20 μm.)