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. 2016 Aug 23;113(36):10204–10209. doi: 10.1073/pnas.1605483113

Table S2.

Primers used in this study

Primer name Primer sequence 5′–3′
oCS184 GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATG AATATTGTCACGGGGGCC
oCS187 GGGGACCACTTTGTACAAGAAAGCTGGGTCCAAAGCTCGAAGACAAGGATCGA
oCS195 GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGGATATTGTCACCGGTGCC
oCS197 GGGGACCACTTTGTACAAGAAAGCTGGGTCCAAAGCTCGAAGGCAAGGGTC
oCS204 GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGGATATTGTCACGGGTGCC
oCS206 GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGAGCACGGAGACGAGGATCA
oCS211 GGGGACCACTTTGTACAAGAAAGCTGGGTCAGCTATCCCATGCTTATGCTTGAC
oCS212 GGGGACCACTTTGTACAAGAAAGCTGGGTCAGCTATTCCATGCTTATCCACGAC
oCS213 GGGGACCACTTTGTACAAGAAAGCTGGGTCAGCTATGCGATGGTGATTCCTG
oCS214 GGGGACCACTTTGTACAAGAAAGCTGGGTCCCGACAATCGAAATCACCTTTAATC
oCS215 GGGGACCACTTTGTACAAGAAAGCTGGGTCGTGACAATCAAAATCACCTTTAATC
oCS216 GGGGACCACTTTGTACAAGAAAGCTGGGTCCCGACAATCAAAATCACCTTTAATC
oCS317 GGATAAGCATGGAATAGCTCACGAGGAGAAGGACATCAAGAAGGAACTCCA
oCS318 TGGAGTTCCTTCTTGATGTCCTTCTCCTCGTGAGCTATTCCATGCTTATCC
oCS320 GCTCACGCGATCAAGGACGAGAAGAAGGAACTCCAGGAG
oCS321 CTCCTGGAGTTCCTTCTTCTCGTCCTTGATCGCGTGAGC
oCS322 GCGATCAAGGACATCAAGAAGGAAGAGCAGGAGGTGGCTG
oCS323 CAGCCACCTCCTGCTCTTCCTTCTTGATGTCCTTGATCGC
oCS324 AGGAACTCCAGGAGGAGGAGGCTAGGCGTGACAGG
oCS325 CCTGTCACGCCTAGCCTCCTCCTCCTGGAGTTCCT
oCS326 ACATCAAGAAGGAACTCCAGGAGGTGGCTGCTGCGGCTGCCGCGGCCGCGTTCGATGGTATTGCTTCTATTCCTACTGAAG
oCS327 CTTCAGTAGGAATAGAAGCAATACCATCGAACGCGGCCGCGGCAGCCGCAGCAGCCACCTCCTGGAGTTCCTTCTTGATGT
oCS328 GCTAGGCGTGACAGGAACAAGGAGGATGGTATTGCTTCTATTCCT
oCS329 AGGAATAGAAGCAATACCATCCTCCTTGTTCCTGTCACGCCTAGC
Sr50F EcoRI GCATAGAATTCATGAATATTGTCACGGGGGCCATG
Sr50163R Xhol GCTATCTCGAGTCACAAAGCTCGAAGACAAGGATCGATAG
Sr50228R Xhol GCTATCTCGAGTCACCGACAATCAAAATCACCTTTAATCTTG
MLA10F EcoRI GCATAGAATTCATGGATATTGTCACCGGTGCCATTTC
MLA10160R Xhol GCTATCTCGAGTCACAAAGCTCGAAGGCAAGGGTCA
MLA10225R Xhol CGTATCTCGAGTCACCGACAATCGAAATCACCTTTAATCTT
SmaI ccdB Fwd CCCCCCGGGCGACGGATGGTGATCCCCCT
Xba1 NLS-YFPrev GGGTCTAGAGTCAACCTCCTACTTTTCGCTTCTTCTTTGGCTTGTACAGCTCGTCCATGCCGA
Xba1 nls-YFPrev GGGTCTAGAGTCAACCTCCTACCTTTCGCTTAGTCTTTGGCTTGTACAGCTCGTCCATGCCGA
Sr33 Prom NotI-F GCATAGCGGCCGCTTCAAGATGTCAAATTTTAAAAGGGCTATT
Sr33 PromR-ApaI CGTATGGGCCCGAGAGCTCTCTTGATCAATCAGCTGAAAC
Sr33 full length ApaI-R GCTATGGGCCCCTCTGCGTCAGAAATCGGTCCTC
Venus ApaI Link-F GCATAGGGCCCGGCGGTAGCGGCGGAGTGAGCAAGGGCGAGGAGCTGTT
Venus-w-NLS-R EcoRI ATGAATTCTTAACCACCGACCTTTCTCTTCTTTTTTGGTTGCTTGTACAGCTCGTCCATGCCGAG
Venus-m-nls-R EcoRI ATGAATTCTTAACCACCAACTTTACGAGTTTTTTTAGGTTGCTTGTACAGCTCGTCCATGCCGAG
Venus-m-nes-R EcoRI ATGAATTCTTATTTATTAGCATCAGCACCAGCAGCTTTAAGAGCAAGTTCATTCTTGTACAGCTCGTCCATGCCGAG
Venus-NES2- EcoRI ATGAATTCTCACTTGTTGATGTCGAGGCCGGCGAGCTTGAGGGCGAGCTCGTTCTTGTACAGCTCGTCCATGCCGAG
Sr33 Term EcoRI-F GCATAGAATTCCTGACGTTGCTTCAGGTGTGCTCTC
Sr33 Term BamHI-R TATGCGGATCCACCTCATTAGGAGGGAGCTGGCG
Venus-full length-R EcoRI ATGAATTCTTACTTGTACAGCTCGTCCATGCCGA
Sr33 attB1-F GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGGATATTGTCACGGGTGC
Sr33 attB2-R GGGGACCACTTTGTACAAGAAAGCTGGGTCCTCTGCGTCAGAAATCGGTC
Sr50 attB1-F GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGAATATTGTCACGGGGGC
Sr50 attB2-R GGGGACCACTTTGTACAAGAAAGCTGGGTCGTTCTCCTCACACAAATCATCATCT
Sr33 genotyping-F GTCCCTTCGCCGTCTGCT
Sr33 genotyping-R GGTCCTCCAACCGGGTAAC
Sr50 F1 TCCCTTCGGCATGATGTCT
Sr50 R1 AAGGGCCCAACCCGGGGTTCTCCTCACACAAATCATCATCA
Sr50 F2 AACCCGGGAAAGTTTAAACTTTATGATGTAGAGGACTCACAGTGAAT
Sr50 R2 AAGGGCCCTGGGCGTGACTGTGCTG
Venus F AACCCGGGGTGAGCAAGGGCGAGGAG
Venus R AAAGTTTAAACTTACTTGTACAGCTCGTCCATGC