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(A) RP-HPLC of the NCR2471-3,2-4 peptide used in a previous study (22) dissolved in Milli-Q water at a concentration of 166 µM. (B) RP-HPLC of NCR2471-3,2-4 peptide dissolved in 10 mM Tris⋅HCl, pH 8.0, at a concentration of 0.5 mg⋅mL−1 and treated with 1 mM TCEP for 15 min. After 15 min, the reaction was quenched by addition of 6% vol/vol TFA in Milli-Q water to afford 2% vol/vol TFA.
