Primers for cloning |
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P. marinus Tbx4/5
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TAYAARTTYGCNGAYAAYAA |
CCYTTNGCRAANGGRTTRTTYTC |
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AARTTYGCNGAYAAYAARTGG |
RAANCCYTTNGCRAANGGRTT |
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CGCGGCTGGTACTTGTGCATCGAG |
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GCGAGTCGGGGTGCACGTAGAG |
Primers for probe synthesis |
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L. erinacea Tbx5
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GGGGAGGTTGTACGTTCACCCAGAT |
GGGCTGGTGAGAAACTGAGGTCTGA |
L. erinacea Flt4
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CGGGACTTCTGGACAATCTCACCGA |
TACTCCACGATCACCATCAGCGGAC |
Danio rerio tbx5a (AF179407) |
CGGTAGACATCGTACAGGCCTCTCC |
ACGGCTTCTTATAGGGGTGTTCTCCTG |
D. rerio flt4 (NM_130945) |
TGCAATGTCAAGCGAGTGAACCCAG |
CAATCTGGATGCCGGGGTATGGAGA |
D. rerio tpma (NM_131105) |
CGCCTTGGACAGAGCAGAGCAG |
AGTGCGATGGAGAAAAGCGGCA |
D. rerio fgf8a (NM_131281) |
TCCTTCACCTCTTTGCGTTT |
CACATCCTGTGCTTCGCTTA |
D. rerio fgf10a (NM_182870) |
ACCAACTCCTCATCGTCTGC |
CAATGTCCGATTCCTCTCGT |
D. rerio col2a1a (NM_131292) |
GGTGATCGTGGTGAGATTGGTGCAC |
CCTCTGTGTCCCTTCTGGCCTCTTT |
P. marinus Tbx4/5
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AARTTYGCNGAYAAYAARTGG |
CCYTTNGCRAANGGRTTRTTYTC |
P. marinus Flt1/4
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GACGAGCGAGGATGAAGGGGAGTAC |
CCACACCATTATCGGCCAGCAGAAC |
(ENSPMAT00000007382) |
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Primers for L. japonicum Tbx4/5 genome sequence |
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Tbx4/5 Gap1 |
CGTGTCGCCTTCCTCCGCAGA |
TGGATGCGTGGGTGATTTCGTGGAT |
Tbx4/5 Gap2 |
CGCCTCCTCCGCTCTCCTTATCTGA |
ACCACGGGAAGAGAGAAAGAGACGC |
Tbx4/5 Gap3 |
TGCGGTCTGTGAACATCGTCACTGG |
AACATCTCCTAGCCACCGTGCTTGC |
Tbx4/5 Gap4 |
TGTGGGAGGAGAAATCGGAGGACCC |
TGCGGTAATTTCCAGAACTGCGGCT |
Tbx4/5 Gap5 |
GCAACTCACGACTACCCCACTTGCA |
GTGGTGACCGTGAGCAGGAATCGAG |
Tbx4/5 Gap6 |
GCAACTCACGACTACCCCACTTGCA |
GTGGTGACCGTGAGCAGGAATCGAG |
Tbx4/5 Gap7 |
GAGGGAGGGAGAGAGAGAGGGACAA |
CGTCACCGGTGTTGGCGGT |
Tbx4/5 Gap8 |
CTGCTGCCCATCACATCACCCGC |
GTGTAGGTGGTCGCGGGCACTTAAG |
Tbx4/5 Gap9 |
CCGTGACCTTTTACATCGTGCACCCAA |
CAACCGGGCAATGACACGATTCAGC |
Tbx4/5 Gap10 |
GTGCTCCTCCTGAACATCGCCCATT |
ACCTAGTTGCTGTACTGCCGTGCTG |
Primers for genomic DNA fragment |
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Mouse Tbx5 intron2 |
CTCCCAGCAAGTCTCCATCATCCCC |
AACTTCAGCCACAGTTCACGTTCATGA |
Mouse CNS12sh |
CCTCATCATCATTAGTCATGCTCGCT |
GCTGCTTCTGGAGACACTGGTC |
Gar Tbx5 intron2 |
TCTCCATCACCACAGACAACATACACTCA |
CCTCCCTGCCTTGGTTATAATCATCTCTGT |
Gar Tbx5 intron5 |
CGCGCGATTGAGGGTTAGAGTTGAA |
AGCGTCTGTTAAGTGATTTCAGATGGCAA |
Gar CNS11 |
AGTCGGCAGAGTCAGCTTCCATAGG |
GTGCTCACTACGCCTTCCATTTCGG |
Gar CNS12 |
GGCTGCTGTCTTAAATACCTTTCTTGGGC |
TGACGGGTGCTATTTGTCCTTTGCC |
Gar CNS12sh |
AAATCATCATTAGTCGTGGCTCATCGTG |
GCCTATCTTGATGTTAGTTTGACCCCTG |
Gar CNS23 |
TCTACAGGCACACACAACCCCTCTG |
CTGGACTCTGGACTCTGGACTGTGG |
Gar CNS26 |
CAGGGATGGAAGGCTTCAAACACTCA |
AGGAATTAAACCCAGGGCCCCAGAG |
Gar CNS27 |
AGGTTACCAGCTACAGTTGTCACACAGATG |
GGCGAGTTCATCCAGTTACACTCAGAAGG |
Gar CNS58 |
AGGCATCGATTCCTTCTCCGTGACG |
ATGTGGACTGCAGGGGTTGTGATGG |
Gar CNS59 |
GGGGAGGCTGTGTGGAAAACTGAGT |
CTTGTAGCACTGGGGCTCTGGGTTC |
Gar CNS63 |
TATAGCGCTCAGTCACGTGGCCTT |
TGCAAATCAACACTCAACGGTACAAGATGT |
Zebrafish tbx5a intron2 |
GTAACGTCAACTAGTGCCTCGATGGT |
CTTTTTAAAGAAGCATGCAGACAGAGAGTGAG |
Zebrafish CNS12sh |
GTGACCTTCTCGCTGTAAGATGATTGT |
ACACATCTTGATTTCATTAACATCTCTTCCTTCT |
Zebrafish CNS65 |
TGTTCCAGGGTCACAATAATGATGCTTTTAAGA |
CATGAAGGTTGCTCTGTGTTCTGCATGA |
SkateTbx5 intron2 |
CCAACAAACCACATTCCCCACAGCA |
TCCCGCTTTCGTAATGATCATCTCTGTCC |
Lamprey DNA fragment |
TCGATGCGTTCCTCAAAAGTGGCAC |
AGGATTAGGGCAGAGGGAGAGGGTG |
Primers for tbx5a mutant genotype |
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Zebrafish tbx5a exon8 |
AGTATTCCTGTGAGAACGGCGTTTCC |
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Zebrafish tbx5a intron8 |
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AGGTATCAGTGTGTGGTTTAGAGGCACA |