Table S2.
List of primers
| Gene/position | Forward | Reverse |
| Primers for cloning | ||
| P. marinus Tbx4/5 | TAYAARTTYGCNGAYAAYAA | CCYTTNGCRAANGGRTTRTTYTC |
| AARTTYGCNGAYAAYAARTGG | RAANCCYTTNGCRAANGGRTT | |
| CGCGGCTGGTACTTGTGCATCGAG | ||
| GCGAGTCGGGGTGCACGTAGAG | ||
| Primers for probe synthesis | ||
| L. erinacea Tbx5 | GGGGAGGTTGTACGTTCACCCAGAT | GGGCTGGTGAGAAACTGAGGTCTGA |
| L. erinacea Flt4 | CGGGACTTCTGGACAATCTCACCGA | TACTCCACGATCACCATCAGCGGAC |
| Danio rerio tbx5a (AF179407) | CGGTAGACATCGTACAGGCCTCTCC | ACGGCTTCTTATAGGGGTGTTCTCCTG |
| D. rerio flt4 (NM_130945) | TGCAATGTCAAGCGAGTGAACCCAG | CAATCTGGATGCCGGGGTATGGAGA |
| D. rerio tpma (NM_131105) | CGCCTTGGACAGAGCAGAGCAG | AGTGCGATGGAGAAAAGCGGCA |
| D. rerio fgf8a (NM_131281) | TCCTTCACCTCTTTGCGTTT | CACATCCTGTGCTTCGCTTA |
| D. rerio fgf10a (NM_182870) | ACCAACTCCTCATCGTCTGC | CAATGTCCGATTCCTCTCGT |
| D. rerio col2a1a (NM_131292) | GGTGATCGTGGTGAGATTGGTGCAC | CCTCTGTGTCCCTTCTGGCCTCTTT |
| P. marinus Tbx4/5 | AARTTYGCNGAYAAYAARTGG | CCYTTNGCRAANGGRTTRTTYTC |
| P. marinus Flt1/4 | GACGAGCGAGGATGAAGGGGAGTAC | CCACACCATTATCGGCCAGCAGAAC |
| (ENSPMAT00000007382) | ||
| Primers for L. japonicum Tbx4/5 genome sequence | ||
| Tbx4/5 Gap1 | CGTGTCGCCTTCCTCCGCAGA | TGGATGCGTGGGTGATTTCGTGGAT |
| Tbx4/5 Gap2 | CGCCTCCTCCGCTCTCCTTATCTGA | ACCACGGGAAGAGAGAAAGAGACGC |
| Tbx4/5 Gap3 | TGCGGTCTGTGAACATCGTCACTGG | AACATCTCCTAGCCACCGTGCTTGC |
| Tbx4/5 Gap4 | TGTGGGAGGAGAAATCGGAGGACCC | TGCGGTAATTTCCAGAACTGCGGCT |
| Tbx4/5 Gap5 | GCAACTCACGACTACCCCACTTGCA | GTGGTGACCGTGAGCAGGAATCGAG |
| Tbx4/5 Gap6 | GCAACTCACGACTACCCCACTTGCA | GTGGTGACCGTGAGCAGGAATCGAG |
| Tbx4/5 Gap7 | GAGGGAGGGAGAGAGAGAGGGACAA | CGTCACCGGTGTTGGCGGT |
| Tbx4/5 Gap8 | CTGCTGCCCATCACATCACCCGC | GTGTAGGTGGTCGCGGGCACTTAAG |
| Tbx4/5 Gap9 | CCGTGACCTTTTACATCGTGCACCCAA | CAACCGGGCAATGACACGATTCAGC |
| Tbx4/5 Gap10 | GTGCTCCTCCTGAACATCGCCCATT | ACCTAGTTGCTGTACTGCCGTGCTG |
| Primers for genomic DNA fragment | ||
| Mouse Tbx5 intron2 | CTCCCAGCAAGTCTCCATCATCCCC | AACTTCAGCCACAGTTCACGTTCATGA |
| Mouse CNS12sh | CCTCATCATCATTAGTCATGCTCGCT | GCTGCTTCTGGAGACACTGGTC |
| Gar Tbx5 intron2 | TCTCCATCACCACAGACAACATACACTCA | CCTCCCTGCCTTGGTTATAATCATCTCTGT |
| Gar Tbx5 intron5 | CGCGCGATTGAGGGTTAGAGTTGAA | AGCGTCTGTTAAGTGATTTCAGATGGCAA |
| Gar CNS11 | AGTCGGCAGAGTCAGCTTCCATAGG | GTGCTCACTACGCCTTCCATTTCGG |
| Gar CNS12 | GGCTGCTGTCTTAAATACCTTTCTTGGGC | TGACGGGTGCTATTTGTCCTTTGCC |
| Gar CNS12sh | AAATCATCATTAGTCGTGGCTCATCGTG | GCCTATCTTGATGTTAGTTTGACCCCTG |
| Gar CNS23 | TCTACAGGCACACACAACCCCTCTG | CTGGACTCTGGACTCTGGACTGTGG |
| Gar CNS26 | CAGGGATGGAAGGCTTCAAACACTCA | AGGAATTAAACCCAGGGCCCCAGAG |
| Gar CNS27 | AGGTTACCAGCTACAGTTGTCACACAGATG | GGCGAGTTCATCCAGTTACACTCAGAAGG |
| Gar CNS58 | AGGCATCGATTCCTTCTCCGTGACG | ATGTGGACTGCAGGGGTTGTGATGG |
| Gar CNS59 | GGGGAGGCTGTGTGGAAAACTGAGT | CTTGTAGCACTGGGGCTCTGGGTTC |
| Gar CNS63 | TATAGCGCTCAGTCACGTGGCCTT | TGCAAATCAACACTCAACGGTACAAGATGT |
| Zebrafish tbx5a intron2 | GTAACGTCAACTAGTGCCTCGATGGT | CTTTTTAAAGAAGCATGCAGACAGAGAGTGAG |
| Zebrafish CNS12sh | GTGACCTTCTCGCTGTAAGATGATTGT | ACACATCTTGATTTCATTAACATCTCTTCCTTCT |
| Zebrafish CNS65 | TGTTCCAGGGTCACAATAATGATGCTTTTAAGA | CATGAAGGTTGCTCTGTGTTCTGCATGA |
| SkateTbx5 intron2 | CCAACAAACCACATTCCCCACAGCA | TCCCGCTTTCGTAATGATCATCTCTGTCC |
| Lamprey DNA fragment | TCGATGCGTTCCTCAAAAGTGGCAC | AGGATTAGGGCAGAGGGAGAGGGTG |
| Primers for tbx5a mutant genotype | ||
| Zebrafish tbx5a exon8 | AGTATTCCTGTGAGAACGGCGTTTCC | |
| Zebrafish tbx5a intron8 | AGGTATCAGTGTGTGGTTTAGAGGCACA | |