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Purification of baseplate wedge complexes using SEC. Samples from fractions across the peak obtained for (A) Mup47/48His6 (pCB14) and (B) Mup46/47/48His6 (pCB85) were analyzed on Coomassie-stained SDS/PAGE gels. His6, hexahistidine tag; Ld, input; MBR, protein molecular mass markers.
