Fig. 4.
Eliminating endogenous MIF significantly enhances the accumulation of misfolded SOD1 at the presymptomatic stage. (A–F) Representative micrographs of lumbar spinal cord sections from SOD1G85R/MIF−/− (A–C) and SOD1G85R/MIF+/+ (D–F) mice at the presymptomatic disease stage, processed for immunofluorescence using the B8H10 antibody, which detects misfolded SOD1 (A and D), and the NeuN antibody, which detects neuronal cells (B and E). (Scale bar: 25 μm.) (G) Quantification of the relative fluorescence intensity of the B8H10 staining of lumbar spinal cord from SOD1G85R/MIF−/− and SOD1G85R/MIF+/+ mice at a presymptomatic disease stage. Approximately 30–35 different areas from different mice of each genotype were analyzed. The bar graph represents mean SEM. ***P < 0.001 (Student’s t test).