Fig. S4.
Deletion of endogenous MIF enhances the mutant SOD1-insoluble fraction in the spinal cords of mutant SOD1G85R mice at disease onset. (A) Schematic drawing of a protocol for testing whether the deletion of endogenous MIF in SOD1G85R mice enhances the accumulation of mutant SOD1 aggregates. (B) Spinal cord extracts were recovered from SOD1G85R/MIF+/+ (WT) and SOD1G85R/MIF−/− (KO) mice at disease onset or in its symptomatic stage. The amount of SOD1 aggregation was determined by immunoblotting of the soluble and insoluble fractions isolated as described in A. Immunoblots were probed with an anti-SOD1 antibody.