Table S2.
Protocol | Genotype | PPR (before Cj) | PPR (after Cj) | n | P (t test) | P (ANOVA) before Cj | P (ANOVA) after Cj |
1 | WT | 1.49 ± 0.04 | 1.50 ± 0.04 | (3) | 0.924 | ||
K882A | 1.42 ± 0.05 | 1.42 ± 0.04 | (4) | 0.992 | 0.575 | 0.341 | |
Δ7 | 1.44 ± 0.03 | 1.47 ± 0.01 | (3) | 0.331 | |||
2 | WT | 1.36 ± 0.02 | 1.38 ± 0.01 | (6) | 0.250 | ||
K882A | 1.39 ± 0.03 | 1.39 ± 0.01 | (3) | 0.707 | 0.136 | 0.390 | |
Δ7 | 1.48 ± 0.06 | 1.43 ± 0.01 | (3) | 0.466 | |||
3 | WT | 1.39 ± 0.03 | 1.39 ± 0.02 | (3) | 0.720 | ||
K882A | 1.34 ± 0.04 | 1.34 ± 0.03 | (3) | 0.888 | 0.208 | 0.504 | |
Δ7 | 1.48 ± 0.07 | 1.46 ± 0.10 | (3) | 0.485 | |||
4 | WT | 1.37 ± 0.03 | 1.39 ± 0.03 | (3) | 0.963 | ||
K882A | 1.34 ± 0.03 | 1.36 ± 0.02 | (3) | 0.391 | 0.253 | 0.362 | |
Δ7 | 1.41 ± 0.01 | 1.42 ± 0.01 | (3) | 0.204 |
PPR was calculated as the mean of the second EPSC-amplitude divided by the mean of the first and EPSC. PPR was compared between before and after Cj, between WT, K882A and Δ7 PCs, and between protocols one and four. No significant difference was detected, suggesting that Cj of any protocol did not affect presynaptic release probability in any of WT, K882A, and Δ7 group. Also no difference was detected in PPR among three genotypes before or after Cj stimulation. Data are shown as mean ± SEM (n).