Table S1.

Summary of all microbubble–cell behaviors, categorized by transmit pulse

Waveform	Type 1	Type 2	Entry at nuc.	Nuc. disp.	Double sono.	PI +	PI −	Total	Entry cyto. only
0.5 MHz, 8-cycle	9	5	6	1	2	23	35	58	5
0.5 MHz, 4-cycle	20	4	2	4	1	31	37	68	1
1 MHz, 8-cycle	17	4	5	4	0	30	27	57	5
2 MHz, 8-cycle	21	6	6	1	2	36	29	65	6
2 MHz, 16-cycle	24	0	4	3	1	32	27	59	8
Total	91	19	23	13	6	152	155	307	25
Total % of PI +	59.4	12.4	15.0	8.5	3.9	100	N/A	N/A	16.3
Total %	29.6	6.2	7.5	4.2	1.9	49.5	50.5	100	8.14

Peak negative acoustic pressures ranged from 0.1 to 0.4 MPa, 0.2 to 0.8 MPa, and 0.2 to 0.6 MPa for 0.5 MHz, 1 MHz, and 2 MHz, respectively. Type 1 and type 2 cytoplasmic PI entry, as well as entry at nucleus (Entry at nuc.), nucleus displacement (Nuc. disp.), double sonoporation (Double sono.), and entry in cytoplasm only (Entry cyto. only) are all defined as illustrated in Fig. S1.