Figure 2. The 3D structure of empty and full OmRV particle.

(A) Raw cryo-EM image of purified OmRV obtained at a nominal magnification of 50,000 with 200 kV accelerating voltage using defocus phase contrast (DPC) cryo-EM. Dotted and solid arrows indicate empty and full virus particles, respectively. The white scale bar represents 50 nm. (B) Resolution plot for the 3D reconstruction of OmRV. The resolution was calculated using the Fourier Shell Correlation (FSC) method of splitting each data set into two halves. The calculated spatial frequencies at 0.143 correlation was 1/8.9 (0.112) Å⁻¹ and at 0.5 correlation was 1/10.1 (0.099) Å⁻¹ for the full particles, and 1/8.3 (0.120) Å⁻¹ and 1/9.6 (0.104) Å⁻¹ for the empty particles. This gives a resolution of 8.9 Å (10.1 Å) and 8.3 Å (9.6 Å), respectively. (C) Stereo views of reconstructed full and empty OmRV structures. The full and empty structures were rendered at an isodensity contour level of 2.1 σ and 2.6 σ, respectively. (D) Cross-sections and (E) radially averaged density profiles of the full and empty particles.