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. 2016 Sep 12;6:31785. doi: 10.1038/srep31785

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Copyright © 2016, The Author(s)

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(a) The selected ion chromatograms of triply charged G37-K74 peptide ion modified with (lower panel m/z 1394.72 shown in red: G37-K74 with GlcNAc) and without GlcNAc (upper panel m/z 1327.02 G37-K74 without GlcNAc) in the LC-MS of API digested of the 20B2-positive fraction (Fig. 1c) is indicated. (b) LC-MS/MS spectrum of triply charged G37-K74 without GlcNAc ion (m/z 1327.02). (c) LC-MS/MS spectrum of triply charged G37-K74 with GlcNAc ion (m/z 1394.72). (d) L1 loop of canonical histone H2A of the mouse. Blue cylinders, a-helical regions; orange cylinder, the L1 loop. The numbers indicate amino acid position.