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. 2016 Sep 12;6:33179. doi: 10.1038/srep33179

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Copyright © 2016, The Author(s)

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(A) Aha1p-mediated ATPase stimulation of Hsp82p^V391E was restored in trans with Hsp82p^D79N (green) but not Hsp82p^E33A (blue). Reactions contained 1 μM Hsp82p^V391E, 10 μM Aha1p and indicated concentrations of Hsp82p^D79N or Hsp82p^E33A. Intrinsic rate of Hsp82p^V391E shown as black stippled line. (B) Aha1p-mediated ATPase stimulation of Hsp82p:Hsp82p^V391E/D79N (green) and Hsp82p:Hsp82p^V391E/E33A (blue) heterodimers. Reactions contained 1 μM Hsp82p, 10 μM Aha1p and indicated concentrations of Hsp82p^V391E/D79N or Hsp82p^V391E/E33A. Intrinsic rate of wildtype Hsp82p shown as black stippled line. (C) Chimera-mediated ATPase stimulation of Hsp82p^V391E was restored in trans with Hsp82p^D79N (green) but not Hsp82p^E33A (blue). Reactions contained 1 μM Hsp82p^V391E, 10 μM Chimera, and indicated concentrations of Hsp82p^D79N or Hsp82p^E33A. Intrinsic rate of Hsp82p^V391E shown as black stippled line. (D) Chimera-mediated ATPase stimulation of Hsp82p:Hsp82p^V391E/D79N (green) heterodimers but not of Hsp82p:Hsp82p^V391E/E33A (blue) heterodimers. Reactions contained 1μM Hsp82p, 10 μM Chimera and indicated concentrations of Hsp82p^V391E/D79N or Hsp82p^V391E/E33A. Intrinsic rate of wildtype Hsp82p shown as black stippled line. All ATPase rates are shown in micromolar ATP hydrolyzed per minute per micromolar of enzyme (1/min).