Figure 2.

Crystal structure of APY-d3 bound to EphA4. (A) Overall structure of the EphA4 LBD (gray, surface representation with loops surrounding the ephrin-binding pocket in darker gray) in complex with APY-d3 (cyan, sticks; PDB 5JR2). (B) Comparison of APY-d3 (cyan, chain E) and APY-d2 (wheat, chain F of PDB 4W4Z).¹⁵ (C) Interactions of APY-d3 βAla1 with EphA4 residues are indicated by green dashes with distances shown in Å. Chains A and E are shown. (D) Representation equivalent to C showing the N-terminus of APY-d2. Black arrows (with distances in Å) indicate that no direct interactions are present between Ala1 of APY-d2 and EphA4. Chains B and F of PDB 4W4Z are shown. All four complexes of the asymmetric unit for both APY-d2 and APY-d3 are shown in Figure S1.