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. 2016 Sep 12;6:33101. doi: 10.1038/srep33101

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Copyright © 2016, The Author(s)

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MG1655(pME6032) (solid line) and MG1655(pME-lasI) (broken lines) were inoculated into MMM + tetracycline, IPTG induced and methionine was added to cultures of MG1655(pME-lasI) at time zero. Selected methionine concentrations (μM) are shown. Strains were grown in an automated microplate reader (TECAN Infinite F200) and changes in cell density (OD₆₀₀) monitored. The data are means ± standard deviations for three independent experiments.