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. 2016 Sep 12;6:33101. doi: 10.1038/srep33101

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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E. coli MG1655(pME6032), E. coli MG1655(pME-lasI) or a 1:1 mixture of the two strains were grown. Colony counts were used to calculate the total population density. To determine the population density of each strain in mixed populations, cfu was assessed (Panel a). The evolutionary fitness of E. coli MG1655(pME-lasI) relative to E. coli MG1655(pME6032) in mixed populations is plotted (Panel b). When the two genotypes have equal fitness, v = 1. Values of v < 1 reflect being outcompeted by MG1655(pME6032 and values >1 indicate that MG1655(pME-lasI) outcompetes MG1655(pME6032). The relative fitness of E. coli MG1655(pME-lasI) in pure culture was calculated by randomly pairing pure MG1655(pME-lasI) and MG1655(pME6032) populations within IPTG treatment.