Figure 1. Experiment 1: Inhibiting Akt1/2 had no effect on overall neuronal differentiation but resulted in the proportion of significantly fewer GABAergic neuron-like cells from P19 mouse embryonal carcinoma cells.

(a) Akt1/2 inhibitor application had no effect on the proportion of differentiated neuron-like cells (Tuj1/GFP, mean ± SEM) derived from P19 cells (Tuj1, neuronal marker; GFP, transfection marker; US2, control plasmid for transfection; Ascl1 (Mash1), inducer of neuronal differentiation; Vehicle, vehicle control; Akt inhibitor, Akt1/2 inhibitor). (b) Akt1/2 inhibitor application resulted in a significant reduction of the proportion of GABAergic neuron-like cells (GAD67/GFP, mean ± SEM) derived from P19 cells (GAD67, GABA marker; GFP, transfection marker). (c) Akt1/2 inhibitor application resulted in a significant reduction in the proportion of parvalbumin-positive neuron-like cells (parvalbumin/GFP, mean ± SEM) derived from P19 cells (GFP, transfection marker). (d) Treatment with the Akt1/2 inhibitor had no significant effect on the proportion of cells with GABA_AR expression on the plasma membrane (GABA_ARs/Na⁺/K⁺ ATPase, mean ± SEM) (Na⁺/K⁺ ATPase, loading control for membrane proteins). *p < 0.05.