Figure 2. Experiment 2: Seizures induced by PTZ (a GABA_AR antagonist and a CNS convulsant) in male and female Akt1^−/− mice and their WT littermate controls.

Measurement of PTZ-induced seizures in WT control mice (white bar) and Akt1^−/− mice (black bar). (a) The latency to initial seizure onset (mean ± SEM, sec) for both males (n = 6 each; Left) and females (n = 8 each; Right). The female Akt1^−/− mice exhibited delayed seizure onset compared to the female WT mice. (b) The average seizure severity score (0–4) (mean ± SEM, sec) for both male (n = 6; Left) and female (n = 8; Right) mice. Seizure severity scores: 0, no signs of motor seizures; 1, isolated twitches; 2, tonic-clonic convulsions; 3, tonic extension; and 4, death. Female Akt1^−/− mice showed lower seizure severity scores than female WT mice. *p < 0.05.