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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cell Signal. 2016 Aug 20;28(11):1789–1805. doi: 10.1016/j.cellsig.2016.08.012

Figure 8. Ectopic overexpression of native CHOP, but not the leucine zipper domain deficient mutant CHOP (CHOP LZ--), evokes a reduction in leptin and IGF1 expression.

Figure 8

Representative western blots (A, B), ELISA immunoassay (C, D), and Real-time RT-PCR analysis (E, F) show that the leucine zipper domain of CHOP that mediates the interaction with C/EBPα is necessary for the CHOP-induced mitigation in leptin and IGF1 expression in treated SH-SY5Y cells (A, C, E) and N2a cells (B, D, F). Luciferase reporter assay demonstrates that only the wild-type native CHOP and not the leucine zipper domain mutant of CHOP attenuates C/EBPα driven leptin and IGF1 promoter transactivation (G, H). Data is expressed as Mean ± S.D and includes determination made in three (n=3) separate cell culture experiments. ***p < 0.001 versus empty vector (EV) – transfected cells.