FIG 3.
Transfer of known or candidate effectors from A. phagocytophilum and W. pipientis through the E. coli pKM101 conjugation channel. (A) E. coli donors produced the TraJ/VirD4Ap or TraJ/VirD4Wp chimera or the respective soluble domains of the VirD4 T4CPs. Transfer of Cre alone, Cre fused to the effector proteins listed, or Cre fused to the C-terminal 100 residues of Ats-1 (CT100) was monitored by CRAfT. The values represent the means of at least three experiments with standard deviations. *, P < 0.01 versus Cre transfer. (B) Steady-state accumulation of Cre or Cre-effector fusion proteins in E. coli donors, detected by immunostaining with anti-Cre antibodies. Lane Vector, donors carrying the vector plasmid. Cre fusion proteins are marked by squares; Cre or prominent Cre breakdown products are marked by rectangles. Cre antibodies also cross-reacted with unknown proteins.