TABLE 3.
Complementation of the pKM101ΔtraJ mutation with genes encoding native and chimeric T4CPs
| Donor transmissible plasmid(s) | Donor complementing plasmid | Transfer frequency for: |
|
|---|---|---|---|
| pKM101 | pML122 | ||
| pKM101 | 2.2 × 10−1 | ||
| pKM101, pML122 | 2.5 × 10−1 | 7.6 × 10−4 | |
| pKM101ΔtraJ | pTB26 (PBAD::traJ) | 2.5 × 101 | 9.9 × 10−3 |
| pKM101ΔtraJ, pML122 | pTB26 (PBAD::traJ) | 2.3 × 102 | 4.3 × 10−3 |
| pKM101ΔtraJ, pML122 | pTB47 (PBAD::virD4At) | ||
| pKM101ΔtraJ, pML122 | pTB48 (PBAD::virD4Ap) | ||
| pKM101ΔtraJ, pML122 | pTB25 (PBAD::virD4Wp) | ||
| pKM101ΔtraJ, pML122 | pNW2 (Plac::traJ/virD4At) | 3.1 × 10−3 | |
| pKM101ΔtraJ, pML122 | pNW (Plac::traJ/virD4K152QAt) | ||
| pKM101ΔtraJ, pML122 | pNW13 (Plac::SDvirD4At) | ||
| pKM101ΔtraJ, pML122 | pTB39 (PBAD::traJ/virD4Ap) | ||
| pKM101ΔtraJ, pML122 | pTB51 (PBAD::traJ/virD4Wp) | ||