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Random mutagenesis of Mm-cpn yielded mutants Mm-cpn-M223I and Mm-cpn-K216E with improved phenotype in E. coli under GroEL- and GroES-depleting conditions. TAB21 cells containing plasmids pET21, pET21-Mm-cpn, pET21-Mm-cpn-M223I, and pET21-Mm-cpn-K216E grown on 0.2% glucose and 1 mM IPTG at 30°C for 5 days.
