FIG 6.
Protease sensitivity test for Mm-cpn-M223I and Mm-cpn-K216E. Protection against proteinase K (PK) for Mm-cpn, Mm-cpn-M223I, and Mm-cpn-K216E as observed by SDS-PAGE. (A) Analysis of Mm-cpn and Mm-cpn-K216E without (−) or with (+) PK in the absence of ATP (lane 1) or in the presence of ATP (lane 2), ADP (lane 3), AMP-PNP (lane 4), or ATP-AlFx (lane 5), all at 1 mM. All gels were stained by Coomassie blue. (B) Analysis of Mm-cpn and Mm-cpn-M223I without (−) or with (+) PK in the absence of nucleotide (lane 1) or in the presence of ATP (lane 2) or ATP-AlFx (lane 3).