TABLE 1.
Analysis of Mm-cpn functionality in ΔgroEL strain AI90 incubated in LB containing sucrose and IPTG
| Temp (°C) | No. of coloniesa for cells expressing: |
|||
|---|---|---|---|---|
| GroEL | Mm-cpn WT | Mm-cpn M223I | Mm-cpn K216E | |
| 26 | +++ | − | − | ++ |
| 30 | +++ | − | − | ++ |
| 37 | +++ | − | − | + |
a
The results are indicative of the average number of colonies obtained for each sample from three independent experiments. The number of cells transformed, amounts of DNA used, and dilutions plated were uniformly maintained to be of equal number. Symbols: −, no colonies; +, between 10 and 50 colonies; ++, between 50 and 100 colonies; +++, between 100 and 200 colonies.