TABLE 5.
Bacteriophage-mediated cotransduction assay using reconstruction of strains with single SNPs for detection of suppression of Δspx lethality
| Allele with SNP | Strain; relevant genotype | Cotransduction frequency (no. of Δspx ermC colonies/total no. of ermC colonies tested) | P valuea |
|---|---|---|---|
| SAOUHSC_01464 | CB1188; P37S mutation | 0/20 | <0.001 |
| AJ862; CB1188 geh::pCL25spx+ | 13/27 | ||
| ytxJ | CB1349; PytxJ* | 0/20 | <0.001 |
| AJ866; CB1349 geh::pCL25spx+ | 11/20 | ||
| rpoB | AJ1008; rpoB+ with kanA nearby | 0/22 | <0.001 |
| AJ1011; AJ1008 geh::pCL25spx+ | 7/13 | ||
| AJ1009; AJ1008 rpoB-P519L with kanA nearby | 14/39 | ||
| AJ1010; like AJ1009 but obtained independently | 9/16 | ||
| WLK8-4R; rpoB-S486L | 0/43 | <0.001 | |
| MV82; WLK8-4R geh::pCL25spx+ | 15/20 | ||
| WLK8-3R; rpoB-D471Y | 4/24 | ||
| WLK8-1R; rpoB-A477V | 5/43 |
a
Exact binomial test. Data are compiled from at least three independent experiments.