FIG 6.

The Sae two-component regulator is necessary for TSST-1 expression in the absence of repression by SarA. Shown are exoprotein profiles (top panel) and Western blot (bottom panel) analysis of TSST-1 for wild-type S. aureus MN8 and the corresponding mutants. Concentrated supernatants from the indicated strains grown in BHI medium were loaded onto 12% SDS-PAGE gels. Molecular mass markers in kilodaltons are shown on the left. Purified recombinant TSST-1 is indicated by the solid arrowhead. The locations of pro-Geh, mature Geh, Aur, ScpA, and nuclease (Nuc) are indicated by open arrowheads.