FIG 7.

BinK is a negative regulator of Syp biofilm phenotypes. Wrinkled colony formation was assessed at 25°C, 28°C, and 30°C in the strains indicated. Only the ΔbinK mutant produced a wrinkled colony at both 25°C and 28°C. Wrinkled colony formation in the ΔbinK mutant was dependent on SypG. Growth curves were obtained for the strains indicated at each of the three temperatures assessed, with the wild-type (WT) curve for each temperature represented by the gray area. Images were taken 48 h postspotting.