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. 2016 Sep 6;11:2139–2147. doi: 10.2147/COPD.S95578

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© 2016 Lea et al. This work is published and licensed by Dove Medical Press Limited

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nSMase-2 and aSMase gene expression in alveolar macrophages.

Notes: RNA was extracted from NS (n=4), S (n=13), and COPD (n=14) alveolar macrophages. Complementary DNA was synthesized by RT-PCR and analyzed for (A) nSMase-2 and (B) aSMase expression using Taqman gene expression primers in a QPCR. Relative expression levels were determined using the ΔCt method normalizing to the endogenous control (GAPDH). Both box and whisker plots illustrate data as medians with the maximum/minimum values out with the upper/lower quartile.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-PCR, reverse transcription polymerase chain reaction; QPCR, quantitative polymerase chain reaction; NS, nonsmoker; S, smoker.