Figure 10.

In vitro evaluation of cytotoxicity of MNPs-NH₂ in (A) HTC after incubation for 2 hours (white columns), 24 hours (red columns), and 48 hours (green columns) in the concentrations of nanoparticles ranging from 5 to 100 mg L⁻¹ by MTT assay and (B) Annexin V/7-AAD flow cytometry; (C) human monocytes for 2 hours (white columns) and 24 hours (red columns) by Annexin-V/7-AAD flow cytometry.

Notes: Healthy cells are Annexin V(−)/7-AAD(−), early apoptotic cells are Annexin V(+)/7-AAD(−), necrotic cells are Annexin V(−)/7-AAD(+), and late apoptotic cells are Annexin V(+)/7-AAD(−). Data are shown as mean ± *P<0.05 vs control.

Abbreviations: 7-AAD, 7-aminoactinomycin D; HTC, hepatoma cells; MNPs, magnetic nanoparticles; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; C, concentration