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. 2016 Sep 6;11:4381–4395. doi: 10.2147/IJN.S114357

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© 2016 Sun et al. This work is published and licensed by Dove Medical Press Limited

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CNTs accelerated cardiogenesis in BASCs and the maturation of BASC-cardiomyocytes (BASC-CMs).

Notes: (A) Immunofluorescent staining of α-SA showing BASCs grown on CNT-Col substrates and Col substrates at days 3, 7, and 10. (B) The percentage of α-SA+ cells of total adherent cells on the two substrates was counted at days 3, 7, and 10. (C) Sarcomere length had significant improvement in BASCs grown on CNT-Col substrates in comparison to those on Col matrix; n=10 cells per group. (D) Z-line width measurements showed there was significant improvement in BASCs grown on CNT-Col substrates when compared to those on Col matrix; n=10 cells per group. (E) Immunofluorescent staining of gap junction protein Cx43 showing BASCs grown on CNT-Col substrates and Col substrates at days 3, 7, and 10. (F) Electron microscopy observation showed BASCs on the CNT-Col substrates possessed better organized cross-striated myofilaments. Z: Z-line; N: nucleus; Mi: mitochondrion. Data are means ± SEM. *Significant difference when compared to control group, *P<0.05. All experiments were performed in triplicate.

Abbreviations: CNT, carbon nanotube; Col, collagen; BASCs, brown adipose-derived stem cells; SEM, standard error of mean; DAPI, 4,6-diamidino-2-phenylindole; N, nucleus; Mi, mitochondrion.

CNTs accelerated cardiogenesis in BASCs and the maturation of BASC-cardiomyocytes (BASC-CMs).

Notes: (A) Immunofluorescent staining of α-SA showing BASCs grown on CNT-Col substrates and Col substrates at days 3, 7, and 10. (B) The percentage of α-SA+ cells of total adherent cells on the two substrates was counted at days 3, 7, and 10. (C) Sarcomere length had significant improvement in BASCs grown on CNT-Col substrates in comparison to those on Col matrix; n=10 cells per group. (D) Z-line width measurements showed there was significant improvement in BASCs grown on CNT-Col substrates when compared to those on Col matrix; n=10 cells per group. (E) Immunofluorescent staining of gap junction protein Cx43 showing BASCs grown on CNT-Col substrates and Col substrates at days 3, 7, and 10. (F) Electron microscopy observation showed BASCs on the CNT-Col substrates possessed better organized cross-striated myofilaments. Z: Z-line; N: nucleus; Mi: mitochondrion. Data are means ± SEM. *Significant difference when compared to control group, *P<0.05. All experiments were performed in triplicate.

Abbreviations: CNT, carbon nanotube; Col, collagen; BASCs, brown adipose-derived stem cells; SEM, standard error of mean; DAPI, 4,6-diamidino-2-phenylindole; N, nucleus; Mi, mitochondrion.