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. 2016 Sep 7;10:2823–2832. doi: 10.2147/DDDT.S105367

Figure 5.

Figure 5

MIC of totarol against S. aureus RN6390.

Notes: The lowest totarol concentration against bacterial growth, after 24 hours incubation at 37°C, was analyzed. The minimal inhibitory concentration was determined to be between 64 µg/mL and 128 µg/mL after 24 hours (A). MIC determination was realized using the marcodilution method with 5×105 bacteria in each tube (B); ** and **** are significantly different from the negative control (P<0.01 and P<0.0001). Results are presented as standard error of the mean (n=6). ADT test on Mueller Hinton blood agar (C). An amount of 10 mg totarol-loaded microspheres were incubated in 1 mL PBS. A filter paper disk was soaked with 20 µL of the supernatant after 7 days of incubation and placed on agar plate. The small but clear inhibition zone showed the antimicrobial activity.

Abbreviations: MIC, minimal inhibitory concentration; S. aureus, Staphylococcus aureus; PBS, phosphate-buffered saline; ADT, agar diffusion test; OD, optical density; EtAc, ethyl acetate.