. Author manuscript; available in PMC: 2017 Feb 25.

Published in final edited form as: Nature. 2016 Aug 25;536(7617):431–436. doi: 10.1038/nature19083

Extended Data Table 3. Summary of cryo-EM data and statistics.

a. Statistics of all cryo-EM reconstructions

Samples	Micrographs collected	Particles used for final reconstruction		Resolution (Å)
APC/C^MCC-Closed	20,234	155,263		4.2
APC/C^MCC-Open		6,188		8.6
APC/C^UbcH10-MCC	2,340	8,491		8.9
APC/C^ΔApc15-MCC	5,054	163,308		4.8

b. Statistics of all cryo-EM structure determination

Data collection
EM		FEI Polara, 300K eV
Detector		FEI Falcon III
Pixel size (Å)		1.36
Defocus range (µm)		2.0-4.0

Reconstruction		APC/C^MCC-Closed	APC/C^MCC-Open	APC/C^UbcH10-MCC	APC/C^ΔApc15-MCC
Software		RELION 1.4	RELION 1.4	RELION 1.4	RELION 1.4
Accuracy of rotation (degrees)		1.318	2.594	3.235	1.61
Accuracy of translations (pixels)		0.843	1.763	2.09	1.08
Final resolution (Å)		4.2	8.6	8.9	4.8

Refinement		APC/C^MCC-Closed
Software		RefMac 5.8
Refmac weight		0.04
Resolution limit (Å)		4.0
Residue number		9,014
Average Fourier shell correlation		0.761
R factor		0.2505
Rms bind length (Å)		0.0126
Rms bond angle (°)		1.746

Validation
Ramachandran plot
Preferred		8427 (93.49%)
Allowed		353 (3.92%)
Outliers		234 (2.60%)