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. Author manuscript; available in PMC: 2016 Sep 12.
Published in final edited form as: Dev Cell. 2014 Nov 20;31(5):599–613. doi: 10.1016/j.devcel.2014.10.012

Figure 6. Tissue contractions are required for successful cell expulsions from the neuroepithelium after wounding.

Figure 6

(A) Complete cell expulsion, where the entire cell body of one cell is removed from the tissue (upper panel) and incomplete cell expulsion, where part of the cell body remains in the tissue (lower panel). Scale bar represents 10 µm.

(B,C) Blocking tissue contractions with Y-27632 (B) or blebbistatin (C) reduces the proportion of complete cell expulsions. CE, complete expulsion. IE, incomplete expulsion. n ≥ 8 animals (B) and n ≥ 23 animals (C) in each condition.* p < 0.05 in Fisher’s exact test.

(D,G) Correlation of contraction amplitude with expulsion speed (D) and size (G). n = 23 animals. r, Pearson’s correlation coefficient.

(E,H) Expulsion speed (E) and size (H) are reduced in the presence of Y-27632. n ≥ 8 animals in each condition. ** p < 0.01 in Student’s t test.

(F,I) Expulsion speed (F) and size (I) are reduced in the presence of blebbistatin. n ≥ 23 animals in each condition. ** p < 0.01 in Student’s t test.

All population data are represented as mean ± sem.

See also Figure S6.