Fig 1. Ischemia-reperfusion experimental design.
Dotted lines represent time course of natriuretic peptide and/or antagonist perfusion. Thin solid lines show regional or global ischemia and full solid lines show stabilisation and reperfusion periods. For infarct size and hemodynamic experiments, all hearts underwent 30 min of regional or global ischemia followed by 90 min reperfusion. L2 (200 nM) or BNP (10 nM) were administered at the time of reperfusion and drug perfusion was maintained for 20 min. For inhibitor study, the natriuretic peptide receptor antagonist isatin (100 μM) was perfused 5 min before regional ischemia until 20 min reperfusion. Mitochondrial ATP-sensitive potassium channel (mitoKATP) blocker, 5 hydroxydecanoate (5-HD, 10 μM) was perfused 5 min before reperfusion for 20 min. For signaling pathway studies and mitochondrial permeability transition pore opening assay, hearts underwent either sham operation or 30 min of regional ischemia followed by 20 min reperfusion.