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. 2016 Sep 12;12(9):e1005834. doi: 10.1371/journal.ppat.1005834

Fig 4. vDCP-NBs are formed in the absence of functional ICP4 and ICP0 in cultured primary mouse TG neurons, and can be associated with viral transcription.

Fig 4

(A) Neurons were infected with in1374 at 32°C for 24 h. (i) IF detection of ICP4, (ii and iii) immuno-FISH for detection of ICP4 or ICP8 and HSV-1 genome, and (iv–vii) immuno-FISH for the detection of PML, Daxx, ATRX or SUMO-2/3 and the HSV-1 genome. (B) and (C) Neurons were infected at 38.5°C for 48 h with in1374 and in1330, respectively. (i–iv) immuno-FISH for the detection of PML, Daxx, ATRX or SUMO-1 and the HSV-1 genome. (D) RT-qPCR for the detection of LacZ transcripts in in1374 infected neurons treated or not with trichostatin A (TSA). Results show means (± SD) of two independent experiments. (E) RNA-DNA FISH combined with IF for detection of LacZ transcripts (blue), HSV-1 genomes (red), and PML (green) in in1374 infected neurons treated or not with TSA. Three different patterns are shown. (F) RNA-DNA FISH combined with IF for detection of LAT transcripts (blue), HSV-1 genomes (red), and PML (green) in mouse TG neurons at 6 to 8 dpi. Scale bars represent 10 μm.