Table 1. Characterization of EV-A71 infectious clones by virus titers and plaque size.

EV-A71 clones	P0 viral titer (log10 PFU/ml)*	Plaque size (mm)*
pT7-EV71	5.80 ± 0.16	1.8 ± 0.5
pCMV-EV71	6.44 ± 0.07	2.2 ± 0.8
pCMV-HH-EV71	5.30 ± 0.25 (P < 0.05)a	2.1 ± 0.6
pCMV-EV71-HDV	7.29 ± 0.27 (P < 0.05)a	2.2 ± 0.6
pCMV-HH-EV71-HDV	5.06 ± 0.27 (P < 0.05)a	2.0 ± 0.4
WT	N.A	2.6 ± 1.1

* The data are presented as means ± standard deviation of at least two biological replicates.

^a Statistically significant compared with pCMV-EV71.

N.A indicates not applicable.