Fig 6. Mutations K261A, E315Q and R372E abolish the full function of LRD6-6 in rice.
Expression of Lrd6-6K261A (A), Lrd6-6E315Q (B) or Lrd6-6R372E (C) in the lrd6-6 mutant genetic background was not able to rescue the autoimmunity and cell death phenotype of lrd6-6. For each transformation, leaves (a) from four independent lines with gene expression verified (b) are shown. Of them, two lines were subjected to cell death (c) and PR genes expression analyses (d), respectively. The relative expressions of the genes were normalized to the Ubq5 reference gene. The error bars represent the SDs of three biology repeats and the expression differences was determined by Student’s t-test (*, P < = 0.05; **, P < = 0.01).